Many pharmaceuticals deployed in the clinic (ex. antibiotics and cytostatics) derive from natural products that are assembled in an iterative fashion by non-ribosomal peptide synthetases (NRPS) and accessory tailoring enzymes. We are interested in understanding the remarkable diversity of non-ribosomal peptide tailoring reactions andthe molecular basis for enzyme:NRPS interactions in efforts to leverage these catalysts for the development of combinatorial libraries of new natural products.
Makris, T. M., Vu, V. V., Meier, K. K., Komor, A. J., Rivard, B. S., Munck, E., Que, L., and Lipscomb, J. D. (2015) An Unusual Peroxo Intermediate of the Arylamine Oxygenase of the Chloramphenicol Biosynthetic Pathway. Journal of the American Chemical Society 137, 1608-1617
Makris, T. M., Chakrabarti, M., Munck, E., and Lipscomb, J. D. (2010) A family of diiron monooxygenases catalyzing amino acid beta-hydroxylation in antibiotic biosynthesis, Proceedings of the National Academy of Sciences of the United States of America 107, 15391-15396.